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Figure 3 | BMC Molecular Biology

Figure 3

From: Interleukin-4 inhibition of interleukin-1-induced expression of matrix metalloproteinase-3 (MMP-3) is independent of lipoxygenase and PPARγ activation in human gingival fibroblasts

Figure 3

IL-4 suppression of MMP-3 is independent of lipoxygenase activity, but inhibition of lipoxygenase super-induces MMP mRNA. HGF cultures were pre-treated with NDGA at the indicated doses for 1 hour prior to addition of IL-1 (10 ng/ml) and/or IL-4 (10 ng/ml). Total RNA was harvested 12 hours after cytokine treatment, quantitated by real-time PCR and normalized to levels of GAPDH. A) MMP-3. The graph represents data from seven independent experiments performed in triplicate, expressed as mean +/- SEM. B) MMP-1. The graph represents five independent experiments performed in triplicate, expressed as mean +/- SEM. Statistical significance was determined using Student's t-test.

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BMC Molecular Biology

ISSN: 1471-2199

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