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Figure 2 | BMC Molecular Biology

Figure 2

From: Interleukin-4 inhibition of interleukin-1-induced expression of matrix metalloproteinase-3 (MMP-3) is independent of lipoxygenase and PPARγ activation in human gingival fibroblasts

Figure 2

There is no qualitative change in binding to the PPRE/AP-1 site in the presence of IL-4 or pioglitazone. A) HGF were incubated in the presence of IL-1 (10 ng/ml), IL-4 (10 ng/ml), and/or pioglitazone (1 μM) as indicated for 1 hour. Five μg nuclear extract were incubated with a 32P-labeled oligodeoxynucleotide probe containing the putative composite PPRE/AP-1 site from the MMP-3 promoter. P = probe alone, no extract; C = control, no cytokines. B) HFG were incubated in the presence of IL-1 (10 ng/ml) or IL-4 (10 ng/ml for 1 hour. Five μg nuclear extract were incubated with the PPRE/AP-1 site probe in the presence of the indicated antibodies. P = probe, no extract.

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BMC Molecular Biology

ISSN: 1471-2199

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