Figure 1From: Interleukin-4 inhibition of interleukin-1-induced expression of matrix metalloproteinase-3 (MMP-3) is independent of lipoxygenase and PPARγ activation in human gingival fibroblastsPPARγ is present in HGF nuclear extract. Fifteen μg nuclear extract isolated from untreated HGF and HGF treated for 24 hour with IL-1 (10 ng/ml), IL-4 (10 ng/ml), or IL-1 and IL-4 were separated on 10% SDS-PAGE, and transferred to nylon membrane. Proteins were detected with anti-PPAR-γ antibodies followed by chemiluminescence. C = control, no cytokines.Back to article page