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Figure 1 | BMC Molecular Biology

Figure 1

From: Checkpoint effects and telomere amplification during DNA re-replication in fission yeast

Figure 1

Effect of inducing cdc18* on DNA content and cell cycle division arrest. cdc18* was induced in the wild-type (green), cds1 Δ (blue), and rad3 Δ (red) strains by removal of thiamine at 0 hours. (A) Fluorescence microscopy of cells 25 hours after cdc18* induction. Cells are stained with DAPI to show nuclei. Arrows show binucleate rad3 Δ cells, which are evidence for active cell division. (B) Histogram plots of DNA content determined by flow cytometry for 0, 17, 21, and 25 hours after removal of thiamine. (C) Density plots of DNA content (fluorescence intensity, x-axis) versus cell size (forward scatter, y-axis) at 25 hours. Wild-type and cds1 Δ cells are greatly elongated with high DNA content whereas rad3 Δ cells are shorter and have lower DNA content due to cell division during re-replication. The insets show the number of cells out of 10,000 represented by pixels of the indicated color.

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