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Table 7 Distinctions among the three column purification-based protocols used in this study.

From: RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies

Parameter Protocol 1 Protocol 2 Protocol 3
Histological sections 4 sections of 20 μm (80 μm) 1 section of 10 μm (10 μm) 2 sections of 10 μm (20 μm)
Deparaffinization 1 ml xylene 100% (3 min – 55°C) 800 μl d-limonene (5 min – room temperature) 1 ml d-limonene (10 min – room temperature)
Proteinase K incubation 3 hours – 50°C "Overnight" – 55°C 3 hours – 55°C
RNA isolation Solution provide by kit 1 Solution provide by kit 2 0.875 μl mercaptoethanol in 125 μl buffer provide by kit 3
DNase incubation 30 min – 37°C 45 min – 37°C 15 min – 37°C
RNA purification Solutions provided by the kit Solutions provided by the kit Solutions provided by the kit