Parameter | Protocol 1 | Protocol 2 | Protocol 3 |
---|---|---|---|
Histological sections | 4 sections of 20 μm (80 μm) | 1 section of 10 μm (10 μm) | 2 sections of 10 μm (20 μm) |
Deparaffinization | 1 ml xylene 100% (3 min – 55°C) | 800 μl d-limonene (5 min – room temperature) | 1 ml d-limonene (10 min – room temperature) |
Proteinase K incubation | 3 hours – 50°C | "Overnight" – 55°C | 3 hours – 55°C |
RNA isolation | Solution provide by kit 1 | Solution provide by kit 2 | 0.875 μl mercaptoethanol in 125 μl buffer provide by kit 3 |
DNase incubation | 30 min – 37°C | 45 min – 37°C | 15 min – 37°C |
RNA purification | Solutions provided by the kit | Solutions provided by the kit | Solutions provided by the kit |