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Table 6 Comparison of the four protocols for quantity and quality of extracted RNA for only samples that produced biologically-useful RNA (RIN ≥ 1.4) in recently archived months old FFPE samples. Case success is defined for each protocol as the percentage of samples where at least one of three RNA extraction attempts provided a RIN ≥ 1.4.

From: RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies

Months old samples:   Protocol 1 Protocol 2 Protocol 3 Protocol 4
FFPE tissue input   80 μm 10 μm 20 μm 50 μm
Successful extractions   34/42 (81%) 34/42 (81%) 39/42 (93%) 35/42 (83%)
Case success   14/14 (100%) 14/14 (100%) 14/14 (100%) 14/14 (100%)
RNA (pg/μl) Minimum value 76 152 987 34
  Maximum value 185,703 259,012 115,194 2,209,739
  Mean 24,818 64,317 26,843 180,584
  Median 14,781 48,255 15,146 51,245
Minimum RIN of 1.4 Minimum value 1.5 1.6 1.4 1.4
  Maximum value 5.1 2.7 2.6 4.9
  Mean 2.3 2.1 2.3 2.3
  Median 2.3 2.0 2.4 2.2