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Table 4 Overall comparison of the four RNA extraction protocols for quantity (in pg/μl) and quality (RIN) for recently archived months old FFPE samples.

From: RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies

Months old samples:   Protocol 1 Protocol 2 Protocol 3 Protocol 4
FFPE tissue input   80 μm 10 μm 20 μm 50 μm
Successful extractions   36/42 (86%) 34/42 (81%) 42/42 (100%) 35/42 (83 %)
RNA (pg/μl) Minimum value 76 152 672 34
  Maximum value 185,703 259,012 115,194 2,209,739
  Mean 25,133 64,317 26,808 180,584
  Median 14,782 48,255 14,599 51,245
RIN Minimum value 1.1 1.6 1.0 1.4
  Maximum value 5.1 2.7 2.6 4.9
  Mean 2.2 2.1 2.2 2.3
  Median 2.3 2.0 2.4 2.2