Table 4 Overall comparison of the four RNA extraction protocols for quantity (in pg/μl) and quality (RIN) for recently archived months old FFPE samples.
Months old samples: | Protocol 1 | Protocol 2 | Protocol 3 | Protocol 4 | |
|---|---|---|---|---|---|
FFPE tissue input | 80 μm | 10 μm | 20 μm | 50 μm | |
Successful extractions | 36/42 (86%) | 34/42 (81%) | 42/42 (100%) | 35/42 (83 %) | |
RNA (pg/μl) | Minimum value | 76 | 152 | 672 | 34 |
Maximum value | 185,703 | 259,012 | 115,194 | 2,209,739 | |
Mean | 25,133 | 64,317 | 26,808 | 180,584 | |
Median | 14,782 | 48,255 | 14,599 | 51,245 | |
RIN | Minimum value | 1.1 | 1.6 | 1.0 | 1.4 |
Maximum value | 5.1 | 2.7 | 2.6 | 4.9 | |
Mean | 2.2 | 2.1 | 2.2 | 2.3 | |
Median | 2.3 | 2.0 | 2.4 | 2.2 |