Table 3 Overall comparison of the four RNA extraction protocols for quantity (in pg/μl) and quality (RIN) for ten year old FFPE samples.
Ten year old samples: | Protocol 1 | Protocol 2 | Protocol 3 | Protocol 4 | |
|---|---|---|---|---|---|
FFPE tissue input | 80 μm | 10 μm | 20 μm | 50 μm | |
Successful extractions | 36/42 (86%) | 26/42 (62%) | 38/42 (90%) | 30/42 (71%) | |
RNA (pg/μl) | Minimum value | 975 | 1,650 | 1,314 | 9864 |
Maximum value | 73,861 | 165,588 | 137,594 | 314,693 | |
Mean | 15,964 | 51,985 | 29,661 | 107,042 | |
Median | 8,750 | 31,104 | 19,458 | 86,682 | |
RIN | Minimum value | 1.0 | 1.5 | 1.0 | 1.8 |
Maximum value | 2.5 | 5.2 | 4.4 | 6.1 | |
Mean | 1.8 | 2.5 | 2.3 | 2.4 | |
Median | 2.0 | 2.3 | 2.2 | 2.2 |