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Table 3 Overall comparison of the four RNA extraction protocols for quantity (in pg/μl) and quality (RIN) for ten year old FFPE samples.

From: RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies

Ten year old samples:   Protocol 1 Protocol 2 Protocol 3 Protocol 4
FFPE tissue input   80 μm 10 μm 20 μm 50 μm
Successful extractions   36/42 (86%) 26/42 (62%) 38/42 (90%) 30/42 (71%)
RNA (pg/μl) Minimum value 975 1,650 1,314 9864
  Maximum value 73,861 165,588 137,594 314,693
  Mean 15,964 51,985 29,661 107,042
  Median 8,750 31,104 19,458 86,682
RIN Minimum value 1.0 1.5 1.0 1.8
  Maximum value 2.5 5.2 4.4 6.1
  Mean 1.8 2.5 2.3 2.4
  Median 2.0 2.3 2.2 2.2