Figure 2

Neuregulin stimulates Egr2 binding to the Nab2 promoter in primary Schwann cells. Primary rat Schwann cells, untreated or exposed to neuregulin for 2 h, were analyzed by ChIP assay. Formaldehyde-crosslinked chromatin from the two cultures were immunoprecipitated with an antibody for Egr2 (filled bars) or purified control IgG (open bars). Percentage recovery relative to input was determined using quantitative PCR with primers designed for the Nab1, Nab2 and Immunoglobin 2A (IMG2A) promoters. The horizontal lines above the promoter diagrams indicate the positions of the amplicons derived from the primer sets used for ChIP analysis. Means and standard deviations were calculated for duplicate measurements from two independent experiments.