Figure 6
Effect of CdCl 2 on HO-1 mRNA deadenylation and stability. A. NIH3T3 cells were treated as controls or with 2.5 μM CdCl2 for 6 h, and total RNA was assayed for HO-1 mRNA deadenylation. Residual full-length mRNA and the 3' end fragment carrying the poly(A) tail are indicated. FD represents the fully deadenylated 3' fragment of HO-1 mRNA. B. The size of the poly(A) tail was estimated as for Fig. 5. C. A fraction of the samples was used without further modification to assess the half-life of HO-1 mRNA. Quantification in panels B and C shows the fitted decay lines calculated from two independent experiments. Data points show the mean and standard errors.