Figure 3

Effect of increasing concentrations of MMS on HO-1 mRNA turnover and NIH3T3 cell proliferation. A. Cells were treated with the indicated concentrations of MMS for 6 h, followed by the addition of AD and RNA extraction at the indicated time points. HO-1 mRNA was detected by northern blotting and normalized to GAPDH loading controls.B. For each concentration in panel A, the fitted decay lines were calculated using data from three independent experiments and plotted. Data points show the mean and standard errors. C. In parallel experiments viable cells (trypan blue-negative) were counted immediately after the treatment (time 0 h) and at 8 h and 24 h after treatment. The percentage of viable cells shown is relative to 100% cell number at time 0 h in untreated controls. The graph shows the mean and standard error of three independent experiments. * p < 0.05 as compared to untreated controls at the corresponding times.