(a) Phylogenetic (NJ) tree for class PII metalloproteinases of viperid venoms. Dataset (complete ORF) used from  in addition to one isoform (DQ464254) obtained in this study (filled circle). ADAM8 from Danio rerio (Q6PFT3) and ADAM7 (from Mus musculus) were used as outgroup. (b) Alignment of the disintegrin domain of class PII SVMPs showing C233, C235, C246 and C251 (marked in grey) which are proposed to be involved in the formation of both monomeric [M] and dimeric [D] disintegrins in the venom. Only relevant portions of the sequences are shown.