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Table 5 Inter-assay variability. Coefficients of variation calculated from R0 values of 8 PCR reactions. Indicated methods for real-time PCR data analysis were used for the transformation of fluorescence data to the R0 values. Designation of the methods is the same as in Table 2.

From: Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis

Gene/Method

St cur

Comp

SCF

DART ind E

DART av E

Liu&S ind E

Liu&S av E

LinReg ind E

LinReg-Ct av E

IL-1β

12.4

12.4

15.3

79.7

18.3

30.3

17.7

68.8

11.2

IL-6

18.6

17.1

20.5

65.5

17.7

40.2

17.5

53.8

16.2