Table 4 Intra-assay variability. Coefficients of variation calculated from R₀ values of 15 replicate PCR reactions. Indicated methods for real-time PCR data analysis were used for the transformation of fluorescence data to the R₀ values. Designation of the methods is the same as in Table 2.

From: Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis