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Table 3 Parameters of the geNorm software for ACTB, SDHA, and HPRT. The parameters were determined from quantities (R0 values) of the three genes in the RT-PCR template dilutions. M – measure of the gene expression stability, V2/V3 – pairwise variations determining the optimal number of reference genes. Indicated methods for real-time PCR data analysis were used for the transformation of fluorescence data to the R0 values. Designation of the methods is the same as in Table 2.

From: Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis

Parameter/Method

St cur

Comp

SCF

DART ind E

DART av E

Liu&S ind E

Liu&S av E

LinReg ind E

LinReg-Ct av E

M for ACTB

0.159

0.161

0.315

0.598

0.200

0.544

0.213

1.772

0.276

M for SDHA

0.187

0.191

0.293

0.578

0.183

0.538

0.326

1.549

0.246

M for HPRT

0.181

0.165

0.324

0.631

0.226

0.626

0.228

1.349

0.338

V2/V3

0.057

0.060

0.097

0.190

0.071

0.195

0.107

0.564

0.109