Figure 3

IL-1β mRNA expression. Quantities of IL-1β, HPRT, SDHA and ACTB mRNAs (the R₀ values) were determined in each sample using all the tested methods of real-time PCR data analysis. The amount of IL-1β mRNA in each sample was then divided by the normalization factor (geometric mean of HPRT, SDHA and ACTB amounts) of the sample. Values are arithmetical means + SEM, n = 5–8. Statistical significance of the differences between the group of untreated colitic animals (Un) and other groups of animals was assessed with the Mann-Whitney test: * P ≤ 0.05, ** P ≤ 0.01. Methods for real-time PCR data analysis: Stand curv, relative standard curve; Comparat, comparative Ct; SCF, sigmoid curve-fitting; DART ind E, DART-PCR with individual E values; DART av E, DART-PCR with average E values; Liu&S ind E, Liu & Saint-exp with individual E values, Liu&S av E, Liu & Saint-exp with average E values; LinReg ind E, LinRegPCR (using individual E values); LinReg-Ct av E, LinRegPCR combined with Ct (using average E values) Designation of the animal groups: the first columns, control sham animals (Sh); the second columns, untreated colitic animals (Un); the third columns, colitic animals with the treatment A; the fourth columns, colitic animals with the treatment B; the fifth columns, colitic animals with the treatment C