Figure 1

Relative standard curves. The standard curves were generated by the Mx3000P software by plotting cycles at threshold fluorescence (Ct) against the logarithmic values of standard RNA amounts. Quantities of standard RNA were expressed as dilution factors of the RNA preparation (1, 0.5, 0.25, 0.125, 0.0125, 0.00125). Correlation coefficients (square values – r²) and amplification efficiencies (E) are shown. SYBRGreen I fluorescences were corrected with passive reference dye (ROX) fluorescences („dRn“).