Figure 8

Replication of subtelomeric heterochromatin in the presence of HU is restrained by the Rad3- and Cds1-dependent replication checkpoint. Here the microarray results at the ends of the sequenced portions of chromosomes 1 and 2 are shown in comparison with the relative enrichment for methylated histone H3 lysine 9 (H3K9me; gold lines at bottoms of graphs) determined by Cam et al. [42]. The "telomere" probe is from the same region of telomere-associated sequences employed by Kim and Huberman [18] for their analysis of telomere replication timing in wild-type and checkpoint-mutant strains. (A) Left end of chromosome 1. (B) Right end of chromosome 1. (C) Left end of chromosome 2. (D) Right end of chromosome 2. The colored symbols representing origins discovered in other laboratories are sparse (A)-(C) or missing (D) in these subtelomeric regions, because the probes used in the other laboratories frequently did not extend to the ends of the 2006 versions of the sequences for S. pombe chromosomes 1 and 2.