Figure 3

Correlations between our results and published studies of fission yeast replication origins. (A) Rectangular Venn diagrams showing the extents to which previously published locations of potential origins correlate with each other. The number in each box is the number of potential origins in the category represented by that box. The area of each box is proportional to the number in the box. The colors in the left- and right-hand boxes are coded in the following way: magenta represents AT islands, purple represents the origins identified by Feng et al. [34] in wild-type cells, orange represents the origins identified by Feng et al. in cds1 Δ cells, light blue indicates origins identified by Heichinger et al. [14], and red indicates pre-RCs identified by Hayashi et al. [15]. The colors in the middle boxes are intended to be blends of the colors in the left- and right-hand boxes, and they are intended to indicate that the middle box in each diagram represents the set of potential origins that was identified in common by the two studies represented by the left- and right-hand boxes. The left- and right-hand boxes, in contrast, show the numbers of potential origins in the indicated studies that did not co-localize with each other. (B) Correlation between the origin efficiencies determined by Heichinger et al. [14] and us. As described in the text, the signal strength that we determined at the position of each of the origins identified by Heichinger et al. was assigned to one of five categories (ranging from below limit to strong; vertical axis) and plotted as a small black circle against the mitotic efficiency of that origin measured by Heichinger et al. (horizontal axis). In cases where more than one origin had identical efficiencies as scored by us and by Heichinger et al., the size of the circle was proportionally enlarged. (C) Correlation between the strong/early or weak/late classifications of pre-RCs by Hayashi et al. [15] and our classifications of origin strength. The numbers of weak/late pre-RCs from the study of Hayashi et al. that fell into each of our classifications are plotted as blue bars against the classifications. The strong/early pre-RCs are similarly plotted as red bars.