Figure 1
From: Checkpoint independence of most DNA replication origins in fission yeast
Synchronization of fission yeast cells by release from G2 arrest into HU. Three isogenic fission yeast strains – JLP1164 (cdc25-22; green), JLP1257 (cdc25-22 cds1 Δ; blue), and JLP1260 (cdc25-22 rad3 Δ; red) were arrested in G2 phase, then released (at time 0) into the cell cycle in the presence of 15 mM HU. (A) Measurements of septation indices at the indicated times after release. (B) Flow cytometric measurements of DNA content, using a fluorescent DNA-specific stain (Sytox Green), at the indicated times after release. See the text for interpretation.