Table 3 Nucleotide sequences of primers and the PCR product size of GAPDH, ACTB, RBP5 and SCD genes
Gene | Primer sequence | Product (bp) |
|---|---|---|
GAPDH | Forward 5'GCATCGTGGAGGGACTTATGA 3' | 67 |
[GeneBank:U85042] | Reverse 5'GGGCCATCCACAGTCTTCTG 3' | |
ACTB | Forward 5'CGCCATGGATGATGATATTGC 3' | 66 |
[GeneBank:AY141970] | Reverse 5'AAGCGGCCTTGCACAT 3' | |
RBP5 | Forward 5'GACGCAAATGCCAGACCATA 3' | 51 |
[GeneBank:XM_587041] | Reverse 5'CACAAACCAGCTGCTCCTCTT 3' | |
SCD | Forward 5'GCTGCTTGTGCGCAAACA 3' | 72 |
[GeneBank:AB075020] | Reverse 5'TCGGCTCTTAGGTCGGATAAATT 3' | |
Probe 5'CAGCTGTCAAAGAAAAGGGTTCCACGCT 3' |