Table 4 Details of gene-specific RQ-PCR assays
Gene symbol | Gene name | Molecular function | Applied Biosystems assay identifier | Amplicon size (bp) | Slope of inhibition curve | PCR Amplification efficiency (%) |
|---|---|---|---|---|---|---|
ABL | Abelson murine leukaemia viral 1 | non-receptor tyrosine protein kinase | Hs00245443_m1 | 54 | -3.47 | 93.9 |
B2M | Beta-2-microglobulin | defense/immunity protein | 4333766 | 75 | -3.48 | 93.6 |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | oxidoreductase, dehydrogenase | 4333764 | 168 | -3.52 | 92.3 |
GUSB | Glucuronidase, beta | galactosidase | 4333767 | 63 | -3.38 | 97.3 |
HPRT1 | Hypoxanthine guanine phosphoribosyl transferase 1 | glycosyltransferase | 4333768 | 100 | -3.37 | 97.7 |
MRPL19 | Mitochondrial ribosomal protein L19 | protein biosynthesis | Hs00608519_m1 | 72 | -3.14 | 107.7 |
PPIA | Peptidylprolyl isomerase A | isomerase | Hs99999904_m1 | 98 | -3.38 | 97.3 |
PSMC4 | Proteasome 26S subunit, ATPase, 4 | protease, hydrolase | Hs00197826_m1 | 83 | -3.38 | 97.6 |
PUM1 | Pumilio, Drosophila, homolog of, 1 | RNA binding, translation regulation | Hs00982776_m1 | 62 | -3.30 | 100.7 |
RPLP0 | Ribosomal protein, large, P0 | protein biosynthesis | 4333761 | 154 | -3.51 | 92.7 |
TFRC | Transferrin receptor | ion receptor | 4333770 | 130 | -3.56 | 90.9 |
ESR1 | Oestrogen receptor alpha | nuclear steroid receptor | Hs00174860_m1 | 62 | -3.45 | 94.5 |