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Table 4 Details of gene-specific RQ-PCR assays

From: Evaluation and validation of candidate endogenous control genes for real-time quantitative PCR studies of breast cancer

Gene symbol Gene name Molecular function Applied Biosystems assay identifier Amplicon size (bp) Slope of inhibition curve PCR Amplification efficiency (%)
ABL Abelson murine leukaemia viral 1 non-receptor tyrosine protein kinase Hs00245443_m1 54 -3.47 93.9
B2M Beta-2-microglobulin defense/immunity protein 4333766 75 -3.48 93.6
GAPDH Glyceraldehyde-3-phosphate dehydrogenase oxidoreductase, dehydrogenase 4333764 168 -3.52 92.3
GUSB Glucuronidase, beta galactosidase 4333767 63 -3.38 97.3
HPRT1 Hypoxanthine guanine phosphoribosyl transferase 1 glycosyltransferase 4333768 100 -3.37 97.7
MRPL19 Mitochondrial ribosomal protein L19 protein biosynthesis Hs00608519_m1 72 -3.14 107.7
PPIA Peptidylprolyl isomerase A isomerase Hs99999904_m1 98 -3.38 97.3
PSMC4 Proteasome 26S subunit, ATPase, 4 protease, hydrolase Hs00197826_m1 83 -3.38 97.6
PUM1 Pumilio, Drosophila, homolog of, 1 RNA binding, translation regulation Hs00982776_m1 62 -3.30 100.7
RPLP0 Ribosomal protein, large, P0 protein biosynthesis 4333761 154 -3.51 92.7
TFRC Transferrin receptor ion receptor 4333770 130 -3.56 90.9
ESR1 Oestrogen receptor alpha nuclear steroid receptor Hs00174860_m1 62 -3.45 94.5