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Table 1 Cycle threshold (Ct) values of candidate EC genes and ESRI. Among the candidate ECs, MRPL19 and PPIA showed the smallest range in Ct values while GAPDH, HPRT1, and RPLP0 showed the greatest. Candidates fell into two groups in terms of abundance, high (Ct 19–20; B2M, RPLP0, GAPDH and PPIA) and moderate abundance (Ct 23–26; PSMC4, ABL, GUSB, TFRC, PUM1, HPRT1 and MRPL19). ESR1 ranged over 10.35 Ct values

From: Evaluation and validation of candidate endogenous control genes for real-time quantitative PCR studies of breast cancer

Gene symbol Ct Range CtMin. CtMax. Mean Ct ± s.e.m.
MRPL19 4.06 24.79 28.84 26.48 ± 0.15
PPIA 4.4 18.01 22.41 19.74 ± 0.18
B2M 4.67 17.22 21.89 19.13 ± 0.21
PUM1 5.06 22.38 27.44 24.78 ± 0.21
GUS 5.19 21.37 26.55 23.94 ± 0.20
PSMC4 5.69 20.84 26.52 23.32 ± 0.22
ABL 5.89 21.27 27.15 23.75 ± 0.30
TFRC 6.03 21.4 27.42 24.40 ± 0.29
GAPDH 6.88 16.79 23.67 19.71 ± 0.25
HPRT1 7.05 21.57 28.61 24.84 ± 0.27
RPLPO 8.8 16.1 24.9 19.46 ± 0.33
ESR1 10.35 17.10 27.45 21.96 ± 0.34