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Table 3 Bacterial strains and plasmids used

From: The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum

Strain or plasmid Relevant markers, phenotypes, and characteristics Reference or origin
C. glutamicum strains   
RES167 Restriction deficient mutant of C. glutamicum ATCC* 13032, Δ (cglIM-cglIR-cglIIR), Nxr [47]
LG01 RES167 with sugR deletion, after double crossover with pLMJ1, Nxr This work
LG02 RES167 with cg2118 deletion, after double crossover with pLMJ2, Nxr This work
LG03 RES167 with cg2118/sugR double-deletion, after double crossover with pLMJ1 and pLMJ2, Nxr This work
E. coli strains   
ER2566 F-ë-fhuA 2 [lon] ompT lacZ::T7 gene1 gal sulA 11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA 1 [dcm] New England Biolabs
JM109 recA 1, endA 1, gyrA 96, thi, hsdR 17, supE 44, relA 1, Δ(lac-proAB)/F' [traD 36, proAB+, lacIq, lacZ ΔM15] Takara Bio Inc.
LG21 JM109 with expression vector pLGI1 for plasmid isolation, Apr This work
LG31 ER2566 with expression vector pLGI1 for the overexpression of SugR, Apr This work
Plasmids   
pK18mobsac B mobilizable E. coli cloning vector, allows for double crossover in C. glutamicum, sacB, lacZ α, Kmr [50]
pZErO-2 E. coli vector, lac promoter, lacZ α, ccdB lethal gene, Kmr Invitrogen
pTYB1 E. coli expression vector, C-terminal intein tag, T7 promoter, lacI, rrnB T1, Apr New England Biolabs
pLMJ1 pK18mobsacB containing 588 bp sugR deletion fragment (sugR-d1/4), obtained by Eco RI-Bam HI fusion, Kmr This work
pLMJ2 pK18mobsacB containing 1117 bp cg2118 deletion fragment (cg2118-d1/4), obtained by Bgl II-Eco RI fusion, Kmr This work
pLGI1 pTYB1 containing sugR (777 bp), obtained by NdeI-SapI fusion, Apr This work
  1. * American Type Culture Collection