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Table 3 Bacterial strains and plasmids used

From: The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum

Strain or plasmid Relevant markers, phenotypes, and characteristics Reference or origin
C. glutamicum strains   
RES167 Restriction deficient mutant of C. glutamicum ATCC* 13032, Δ (cglIM-cglIR-cglIIR), Nxr [47]
LG01 RES167 with sugR deletion, after double crossover with pLMJ1, Nxr This work
LG02 RES167 with cg2118 deletion, after double crossover with pLMJ2, Nxr This work
LG03 RES167 with cg2118/sugR double-deletion, after double crossover with pLMJ1 and pLMJ2, Nxr This work
E. coli strains   
ER2566 F-ë-fhuA 2 [lon] ompT lacZ::T7 gene1 gal sulA 11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA 1 [dcm] New England Biolabs
JM109 recA 1, endA 1, gyrA 96, thi, hsdR 17, supE 44, relA 1, Δ(lac-proAB)/F' [traD 36, proAB+, lacIq, lacZ ΔM15] Takara Bio Inc.
LG21 JM109 with expression vector pLGI1 for plasmid isolation, Apr This work
LG31 ER2566 with expression vector pLGI1 for the overexpression of SugR, Apr This work
pK18mobsac B mobilizable E. coli cloning vector, allows for double crossover in C. glutamicum, sacB, lacZ α, Kmr [50]
pZErO-2 E. coli vector, lac promoter, lacZ α, ccdB lethal gene, Kmr Invitrogen
pTYB1 E. coli expression vector, C-terminal intein tag, T7 promoter, lacI, rrnB T1, Apr New England Biolabs
pLMJ1 pK18mobsacB containing 588 bp sugR deletion fragment (sugR-d1/4), obtained by Eco RI-Bam HI fusion, Kmr This work
pLMJ2 pK18mobsacB containing 1117 bp cg2118 deletion fragment (cg2118-d1/4), obtained by Bgl II-Eco RI fusion, Kmr This work
pLGI1 pTYB1 containing sugR (777 bp), obtained by NdeI-SapI fusion, Apr This work
  1. * American Type Culture Collection