Figure 6
From: Hepatoma derived growth factor binds DNA through the N-terminal PWWP domain
PWWP domain is required for HDGF DNA binding. EMSA was carried out as described above using truncated recombinant HDGF protein. The bottom panel is a schematic illustration of the GST-HDGF truncated polypeptides generated to determine the region(s) of HDGF that allow for binding with the 80 bp DNA probe. The PWWP domain is located at amino acids 1–90. NLS represents the location of the HDGF nuclear localization sequence.