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Table 1 ΔG of SD:aSD interactions.

From: Translation initiation region sequence preferences in Escherichia coli

Shine-Dalgarno ΔG37°C ΔG20°C
  (kcal/mol) (kcal/mol)
UAAGGAGG -9.4 -12.6
AAAGGAGG -9.3 -12.3
AUAGGAGG -7.7 -10.1
AUUGGAGG -6.9 -9.4
AUUCGAGG -3.9 -5.7
AUUGGAG C -4.7 -6.7
AUUCCAGG -1.0 -2.1
AUUCGAG C -1.7 -2.9
AUUCCUGG -0.1 -1.3
AUUCCUCG NA NA
  1. ΔG of SD:aSD interactions at 37°C and 20°C, including 8 nucleotides from mRNA and 8 nucleotides from 16S rRNA. Free energy was calculated using hybrid-min from the UNAFold package [45].