BMC Molecular Biology

Table 1 General expression levels of the candidate 'housekeeping' genes in fathead minnow liver and gonad tissues.

From: Appropriate 'housekeeping' genes for use in expression profiling the effects of environmental estrogens in fish

LIVER		GONAD
'Housekeeping' Gene	C_t (Mean ± S.E.)	'Housekeeping' Gene	C_t (Mean ± S.E.)
18S rRNA	11.72 ± 0.35	18S rRNA	11.58 ± 0.32
ef1a	22.55 ± 0.18	bactin	23.24 ± 0.29
gapdh	23.95 ± 0.41	ef1a	23.34 ± 0.43
rpl8	25.65 ± 0.51	rpl8	24.78 ± 0.65
bactin	28.12 ± 0.64	tbp	25.74 ± 0.24
g6pd	28.40 ± 0.50	gapdh	27.26 ± 0.37
hprt1	29.51 ± 0.67	hprt1	28.68 ± 0.22
tbp	31.96 ± 0.56	g6pd	29.76 ± 0.24

Genes are shown from highest expressed (low C_t, top) to lowest expressed (high C_t, bottom) for each tissue based on mean real-time PCR C_t values in control fish.

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ISSN: 1471-2199

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