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Figure 3 | BMC Molecular Biology

Figure 3

From: Appropriate 'housekeeping' genes for use in expression profiling the effects of environmental estrogens in fish

Figure 3

Expression of the gene of interest vtg in fathead minnow liver following exposure to 10 ng EE 2 /L. vtg expression data is presented (A) normalized to input cDNA, or normalized to the expression of different 'housekeeping' genes: (B) 18S rRNA, (C) rpl8, (D) ef1a, (E) g6pd, (F) bactin, (G) gapdh, (H) hprt1, and (I) tbp, measured in each sample. The results are represented as means ± S.E.M., expressed as fold-increase in expression from the control which was arbitrarily set as the 1× expression of the gene, and shown on a logarithmic scale. Each treatment group consisted of eight male and eight female fish and each fish was analysed in triplicate. Statistically significant differences in gene expression between control and EE2-treated fish for each sex are denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001 (Student's t-test).

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