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Figure 4 | BMC Molecular Biology

Figure 4

From: Characterization of novel elongated Parvulin isoforms that are ubiquitously expressed in human tissues and originate from alternative transcription initiation

Figure 4

Detection of Par17 in cell lysates. A. Western blot of HeLa and HepG2 cell lysates. 10 and 50 μg HeLa (lane 1 and 2) and 10 and 50 μg HepG2 (lane 3 and 4) cell extracts were separated by SDS-PAGE with Tris-glycine as running buffer and transferred to nitrocellulose membranes. SeeBlue2 was used as protein standard. Blots were incubated with Ab-PPIase (polyclonal antibody against Parvulin's PPIase domain as described [11,12], stripped by 2% SDS at 65°C and re-probed with affinity purified Ab-EXT (against the N-terminal extension). Coomassie stained membrane is shown as loading control. B. Fresh protein samples (lane 1) as well as samples subjected to repeated freeze-thaw cycles (lane 2) were analyzed by Western blots using anti-Par17 antiserum. C; HeLa cell extracts were separated by SDS-PAGE with Tris-glycine as running buffer, transferred to nitrocellulose and incubated with antibodies against SUMO-1 (lane1), SUMO-2/3 (lane2) and Ubiquitin (lane3). Magic Mark was used as protein standard.

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BMC Molecular Biology

ISSN: 1471-2199

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