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Table 2 Oligonucleotides used for EMSA for putative binding sites

From: Cooperation between MEF2 and PPARγ in human intestinal β,β-carotene 15,15'-monooxygenase gene expression

Oligonucleotide sequencea

DNA element

5'-GCTTGGAAATTAACCTTTAACCA AACAT-3'

Wild-type PPAR

5'-GCTTGGAAATTAtgCTTTAtgCA AACAT-3'

Mutated PPAR

5'-GGATTAAAGATTAGC TATGACACCATG-3'

Wild-type GATA-1

5'-GGATTAActcTTAGC TATGACACCATG-3'

Mutated GATA-1

5'-GGTTAAGTCAAAAGTG AAACTGGAG-3'

Wild-type IRF-1

5'-GGTTAAGTCAtcAGTG AAACTGGAG-3'

Mutated IRF-1

5'-CTCTCTGCTTATTTAG AACCTAGTC-3'

Wild-type MEF2

5'-CTCTCTGCTTgTTTAc AACCTAGTC-3'

Mutated MEF2

5'-GAACTCCTGACCT CAAGTGACCC GCCAC-3'

Wild-type CRE/RAR

5'-GAACTCCTGtgCT CAAGTGtgCC GCCAC-3'

Mutated CRE/RAR

  1. aUpper strands of oligonucleotides with putative consensus sequences are underlined; the mutated nucleotides are in lower case characters.
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