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Table 1 Sequences1 of oligonucleotides used

From: Length-dependent degradation of single-stranded 3' ends by the Werner syndrome protein (WRN): implications for spatial orientation and coordinated 3' to 5' movement of its ATPase/helicase and exonuclease domains

C50

GCTGATCAACCCTACATGTGTAGGTAACCCTAACCCTAACCCTAAGGACA

C55

GCTGATCAACCCTACATGTGTAGGTAACCCTAACCCTAACCCTAAGGACAACCCT

C60

GCTGATCAACCCTACATGTGTAGGTAACCCTAACCCTAACCCTAAGGACAACCC-TAGTGA

C65

GCTGATCAACCCTACATGTGTAGGTAACCCTAACCCTAACCCTAAGGACAACCCTAGTGAAGCTT

C70

GCTGATCAACCCTACATGTGTAGGTAACCCTAACCCTAACCCTAAGGACAACCCTAGTGAAGCTTGTAAC

G24 2

CCTACACATGTAGGGTTGATCAGC

G30

GGGTTACCTACACATGTAGGGTTGATCAGC

G35

GGTTAGGGTTACCTACACATGTAGGGTTGATCAGC

G40

GTTAGGGTTAGGGTTACCTACACATGTAGGGTTGATCAGC

G45

TTAGGGTTAGGGTTAGGGTTACCTACACATGTAGGGTTGATCAGC

G80

CTTCACAGTCAGAGTCACAGTGTGCCGGGTTGTCCTTAGGGTTAGGGTTAGGGTTACCTACACATGTAGGGTTGATCAGC

1All sequences are depicted in 5' to 3' orientation.

2For EMSA on single-stranded G24, G30, G35, G40, G45. and G80, each oligomer included a 3'-PO4 moiety to block WRN exonuclease activity; overhang substrates were constructed with G45 with a 3' PO4 group, also to block potential WRN exonuclease activity on the unlabeled strand.

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BMC Molecular Biology

ISSN: 1471-2199

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