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Figure 6 | BMC Molecular Biology

Figure 6

From: Length-dependent degradation of single-stranded 3' ends by the Werner syndrome protein (WRN): implications for spatial orientation and coordinated 3' to 5' movement of its ATPase/helicase and exonuclease domains

Figure 6

Effect of various ribonucleotide cofactors on length-dependent WRN exonuclease activity. A) Wild type WRN (2 nM) was incubated with labeled G35 (0.1 nM) for 10 min at 37°C without or with 1 mM ATP (A), GTP (G). CTP (C), or UTP (U) as indicated. DNA products were analyzed as described for the exonuclease assay in Methods. B) Wild type WRN (2.7 nM) was incubated with the 10 nt 3' overhang (labeled C55/G45) substrate (0.25 nM) for 10 min at 37°C without or with 1 mM ATP (A), GTP (G), CTP (C), or UTP (U). Reactions were analyzed in parallel for exonuclease (top panel) and unwinding (bottom panel) activities as described in Fig. 5B; heat-denatured substrate was used as a marker for single-stranded C55 (lane 7, bottom panel).

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