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Figure 2 | BMC Molecular Biology

Figure 2

From: The in vitro real-time oscillation monitoring system identifies potential entrainment factors for circadian clocks

Figure 2

Endogenous clock gene expression profiles stimulated by 15d-PGJ 2 . NIT3T3 cells were stimulated by 50% serum (positive control; left column), DMSO (negative control; right column), or 10 μM 15d-PGJ2 (middle panel) for 1 h. Total RNAs were isolated at each time point. Quantitative real-time RT-PCR was performed using mPer2 (upper panels), mBmal1 (bottom panels), and 18S rRNA primers. Abscissa presents "hour", ordinate "mRNA amount", respectively. mRNA amount at time 0 was set to 1. The relative levels of each mRNA were normalized to the corresponding 18S rRNA levels.

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