Table 1 Summary of proteins identified by peptide mass fingerprint and peptide sequencing
Band (molecular weight) | Proteins, abbreviation, and SwissProt accession number | Number of sequenced peptides |
|---|---|---|
1 (110 kDa) | - Poly (ADP-ribose) polymerase 1 (PARP-1; P09874) | 3 (m) |
2 (75 kDa) | - ATP-dependent helicase II (Ku80; P13010) | 1 (m) |
3 (65 kDa) | - ATP-dependent helicase II (Ku70; P12956) | 2 (m) |
4 (60 kDa) | - PARP-1 - Ku70 - Keratin | 2 (LC) 2 (LC) |
5 (50 kDa) | - PARP-1 - Keratin | 6 (LC) |
6 (35 kDa) | - DNA-(apurinic or apyrimidine site) lyase (APEX1; P27695) - Glyceraldehyde-3-phosphate hydrogenase (GAPDH; P04406) | 9 (LC) 3 (LC) |
7 (25 kDa) | - PARP-1 | 9 (LC) |