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Table 1 Integration efficiency of vectors carrying attP core modifications

From: Modification of the mycobacteriophage Ms6 attP core allows the integration of multiple vectors into different tRNAala T-loops in slow- and fast-growing mycobacteria

Vector 26 bp core attPa Integration efficiency/pAV6950b   % integration into the tRNAalaT-loopc  
   M. smegmatis M. bovis BCG M. smegmatis M. bovis BCG
pAV6950 AGGGGTTCGAATCCCCTTAGCTCCAC 100% 100% 100% in tRNAalaU 100% in tRNAalaV
pBU AGGGGTTCGAG TCCCCTTAGCTCCAC 1.1% 34% ND 100% in alaU
pBT AGGGGTTCGAG TCCCCTAG GCTCCAC 0.7% 18% ND 100% in tRNAalaT
pSV AGGGGTTCGAT TCCCCTTAGCTCCAC 12% 0.8% 100% tRNAalaV ND
pST AGGA GTTCGAATCT CCTAG GCTCCAC 0.05% ND ND ND
  1. a: Changes introduced by site-directed mutagenesis, with respect to the original attP site from pAV6950 are indicated in bold
  2. b: Identical amounts of plasmid DNA were used to electroporate competent M. smegmatis or BCG. Integrants were numbered and the percentage of [integrants with modified attP/integrants with pAV6950] was calculated.
  3. c: The insertion locus was determined by PCR amplification and the percentage of clones in the given tRNAala T-loop was calculated