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Figure 1 | BMC Molecular Biology

Figure 1

From: Modification of the mycobacteriophage Ms6 attP core allows the integration of multiple vectors into different tRNAala T-loops in slow- and fast-growing mycobacteria

Figure 1

Southern blot of genomic DNA from wild-type and pAV6950-transfected M. bovis BCG and M. smegmatis integrants. A: Alignment between the three tRNAala gene sequences from M. bovis BCG or M. smegmatis and the Ms6 att P 26-base core sequence. The coloured blocks represent identical sequence stretches, base differences are indicated in bold and the anticodon is underlined. The position of anticodon and T-loops in the tRNAala sequence is indicated. B: Genomic DNA from wild-type BCG (lane 1) and a pAV-SIV integrant (lane 2) was probed with a PCR product based on the tRNAalaU (left panel) or tRNAalaV (right panel) sequence from the M. tuberculosis genome. C: Genomic DNA from wild-type M. smegmatis (lane 1) and a pAV-SIV integrant (lane 2) was probed with a PCR product based on the tRNAalaU (left panel) or tRNAalaV (right panel) sequence from the M. smegmatis genome.

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