Table 1 Summary of results for mTARC putative STAT6 sites
Putative site | Position within mTARC promoter | Sequence | Significance inferred from 5 'deletion series | Binds STAT6 in EMSA | Trimer construct induced by IL-4 in transfections | Reduction in IL-4 induction of mTARC -1173 when site mutated (approx.) |
|---|---|---|---|---|---|---|
T1 | -140 to -148 | TTC TTTGAA* | + | + | - | 85% |
T2 | -194 to -203 | TTC TCTGGAA | + | + | ++ | 100% |
T3 | -473 to -482 | ATC CCTGTAA | - | - | ND | ND |
T4 | -484 to -493 | TTC TACTGAA | - | + | + | 40% |
T5 | -513 to -522 | TTC CCCAAAA | + | - | ND | 60% |
T6 | -531 to-540 | TTC AGCTGAA | + | - | - | 60% |
T7 | -657 to -666 | TT TCTGGGA G | - | - | ND | ND |
T8 | -763 to -111 | TTC TAGGGCA | - | - | ND | ND |
T9 | -854 to -863 | TTC TGGGCA C | - | - | ND | ND |
T10 | -870 to -879 | TT GTCCTGAA | - | - | ND | ND |
T11 | -904 to -913 | TTC TCAGTAA | - | + | ++ | 15% |