Figure 7

Site-directed mutagenesis reveals a critical role for T1 and T2 and significant contributions from T4, T5 and T6 sites in the IL-4 induction of mTARC -1173. RAW 264.7 cells were transfected as described in Figure 4A with 5 μg luciferase-conjugated mutant constructs (T1-11 mutant) and 3 μg STAT6 expression vector. Data are representative of at least 2 transfections and results are displayed as fold induction of sample relative luciferase activity (luciferase/β-galactosidase) with cytokine treatment +/- SD.