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Figure 4 | BMC Molecular Biology

Figure 4

From: Interleukin-4 induction of the CC chemokine TARC (CCL17) in murine macrophages is mediated by multiple STAT6 sites in the TARC gene promoter

Figure 4

The eleven putative STAT6 sites within the proximal mTARC promoter contribute differentially to the IL-4 induction. Ten million RAW 264.7 cells were co-transfected with 5 μg mTARC promoter 5' deletion luciferase constructs and 3 μg STAT6 expression vector, β-galactosidase expression vector (1 μg) was also co-transfected to control for transfection efficiency. Identical transfections were pooled and plated into duplicate wells for each treatment. Cells were treated +/- 20 ng/ml recombinant murine IL-4 for 16–18 hours before being harvested and assayed for luciferase and β-galactosidase activity. Data are representative of 2 transfections and results are expressed as Luciferase Activity normalised for β-galactosidase activity +/- SD. Deletion construct end points are marked and correspond to the sequence information contained in Figure 3. The putative STAT6 sites contained in each construct are also indicated.

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