Biochemical characterisation of LigN, an NAD+-dependent DNA ligase from the halophilic euryarchaeon Haloferax volcanii that displays maximal in vitro activity at high salt concentrations

Table 4 Oligonucleotide primers used in this study.

Oligonucleotide	Sequence (5'-3')
HVOLIGN-KF	GATTACGTCTGCGAACCCGCGTTCGACGGCCTCTCGGTC
HVOLIGN-KR	GACCGAGAGGCCGTCGAACGCGGGTTCGCAGACGTAATC
HVOLIGN-DF	GTCTGCGAACCCAAGTTCGCCGGCCTCTCGGTCGAAGTC
HVOLIGN-DR	GACTTCGACCGAGAGGCCGGCGAACTTGGGTTCGCAGAC
HVOLIGN-PCR-1R	CGGTGACGCGGAGACCCCACTCGC
PET47B-5SEQ	GCCTAGGTTAATTAAGCCTCGAGA
HVOLIGN-N-5BAM	GTTGGTTGGTggatccGATGAGCGACGCCGACGTCGACGC
HVOLIGN-N-3H	GTGTGTGTGTaagcttCTACCCGCCGCCGTCTTCGGTGAC

Note: Sequencing primers are not shown but details are available from the authors on request. Restriction sites are shown in lower case.

ISSN: 1471-2199