Biochemical characterisation of LigN, an NAD+-dependent DNA ligase from the halophilic euryarchaeon Haloferax volcanii that displays maximal in vitro activity at high salt concentrations

Table 2 Pairwise primary sequence identity (%) between haloarchaeal ATP-dependent DNA ligases.

	Hvo	Hma	Nph	Hsa	Hqw 1	Hqw 2
Hvo	100	65.4	66.3	65.8	53.8	33.1
Hma	65.4	100	67.5	64.6	50.3	35.6
Nph	66.3	67.5	100	69.5	55.2	37.3
Has	65.8	64.6	69.5	100	51.9	36.3
Hqw1	53.8	50.3	55.2	51.9	100	31.2
Hqw2	33.1	35.6	37.3	36.3	31.2	100

Abbreviations: Hvo (Haloferax volcanii), Hma (Haloarcula marismortui),Nph (Natronomonas pharaosis), Hsa (Halobacterium salinarum), Hqw 1 (Haloquadratum walsbyi HQ2659A protein) and Hqw 2 (H.walsbyi HQ2327A protein). Sequence identities were calculated using default settings with the SIM alignment [41].

ISSN: 1471-2199