Biochemical characterisation of LigN, an NAD+-dependent DNA ligase from the halophilic euryarchaeon Haloferax volcanii that displays maximal in vitro activity at high salt concentrations

Table 1 Pairwise primary sequence identity (%) between haloarchaeal NAD⁺-dependent DNA ligases.

	Hvo	Hma	Nph	Hqw	HF70
Hvo	100	62.1	55.4	60.1	32.1
Hma	62.1	100	61.7	58.2	32.3
Nph	55.4	61.7	100	54.0	31.8
Hqw	60.1	58.2	54.0	100	31.9
HF70	32.1	32.3	31.8	31.9	100

Abbreviations: Hvo (Haloferax volcanii), Hma (Haloarcula marismortui),Nph (Natronomonas pharaosis), Hqw (Haloquadratum walsbyi HQ2659A protein) and HF70 (uncultured marine archaeon HF70_B12). Sequence identities were calculated using default settings with the SIM alignment tool [41]

ISSN: 1471-2199