Figure 5
Phylogenetic analysis of archaeal ATP-dependent DNA ligases. Phylogenetic tree showing the evolutionary relationship of 41 archaeal ATP-dependent DNA ligase proteins together with the closely-related enzyme encoded by the eubacterium Aquifex aeolicus. Crenarchaeal species are shown in red, whereas halophilic euryarchaeal species (such as Hfx.volcanii) are shown in blue. The Aquifex enzyme (Aae, indicated in green) can be seen clustering with the hyperthermophilic (non-marine) crenarchaeal species shown at the top of the figure. Archaeal species abbreviations: Hvo (Haloferax volcanii), Aam (Acidianus ambivalens), Afu (Archaeoglobus fulgidus), Ape (Aeropyrum pernix), Csy (Cenarcheon symbiosum), Hma (Haloarcula marismortui),Fac (Ferroplasma acidarmanus), Nph (Natronomonas pharaosis), Hqw (Haloquadratum walsbyi), Has (Halobacterium salinarum), Mac (Methanosarcina acetivorans), Mba (Methanosarcina barkeri), Mbu (Methanococcoides burtonii), Mhu (Methanospirillum hungatei), Mja (Methanocaldococcus jannaschii), Mka (Methanopyrus kandleri), Mma (Methanosarcina mazei), Mmr (Methanococcus maripaludis), Mpt (Methanosaeta thermophila), Mst (Methanosphaera stadtmanae), Mth (Methanothermobacter thermautotrophicus), Neq (Nanoarchaeum equitans), Pab (Pyrococcus abyssi), Pae (Pyrobaculum aerophilum), Pfu (Pyrococcus furiosus), Pho (Pyrococcus horikoshii), Pto (Picrophilus torridus), Sac (Sulfolobus acidocaldarius), Ssh (Sulfolobus shibatae), Sso (Sulfolobus solfataricus), Sto (Sulfolobus tokodaii), Tac (Thermoplasma acidophilum), Tfu (Thermococcus fumicolans), Tko (Thermococcus kodakarensis), Tsp (Thermococcus sp. NA1), Tvo (Thermoplasma volcanium), 74A4 (uncultured crenarchaeote 74A4). The tree was generated using TreeView X [39] from sequence alignment data generated using Clustal X [40]. A full listing of the database accession numbers of the proteins used to construct the tree can be obtained from the authors on request.