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Figure 4 | BMC Molecular Biology

Figure 4

From: Studies on the CPA cysteine peptidase in the Leishmania infantum genome strain JPCM5

Figure 4

Analysis of Licpa null mutants. A) PCR analysis of the two ΔLicpa mutants. PCR reactions were performed using primers indicated in Figure 3A. WT: wild-type, H; heterozygote CPA/ΔLicpa, ΔC1: ΔLicpaC1 and ΔC2: ΔLicp aC2. B) Southern blot analysis of the ΔLicpa C1 (lane A) and ΔLicpa C1::CPA (lane B) re-expresser mutant DNA digested with Pst I and hybridized with a 5'FR probe. C) RT-PCR analysis of wild type (WT), ΔLicpaC1 (ΔC1), ΔLicp aC1::CPA (ΔC1:CPA), ΔLicpaC2 (ΔC2) and ΔLicp aC2::CPA (ΔC2:CPA) parasites. Nested PCR were carried out in presence (+) or in absence (-) of cDNA using the primer pairs OL136/SL primer. Lower panel is a control with the LinJ36.2050 gene.

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