Figure 4From: Studies on the CPA cysteine peptidase in the Leishmania infantum genome strain JPCM5Analysis of Licpa null mutants. A) PCR analysis of the two ΔLicpa mutants. PCR reactions were performed using primers indicated in Figure 3A. WT: wild-type, H; heterozygote CPA/ΔLicpa, ΔC1: ΔLicpaC1 and ΔC2: ΔLicp aC2. B) Southern blot analysis of the ΔLicpa C1 (lane A) and ΔLicpa C1::CPA (lane B) re-expresser mutant DNA digested with Pst I and hybridized with a 5'FR probe. C) RT-PCR analysis of wild type (WT), ΔLicpaC1 (ΔC1), ΔLicp aC1::CPA (ΔC1:CPA), ΔLicpaC2 (ΔC2) and ΔLicp aC2::CPA (ΔC2:CPA) parasites. Nested PCR were carried out in presence (+) or in absence (-) of cDNA using the primer pairs OL136/SL primer. Lower panel is a control with the LinJ36.2050 gene.Back to article page