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Figure 2 | BMC Molecular Biology

Figure 2

From: Studies on the CPA cysteine peptidase in the Leishmania infantum genome strain JPCM5

Figure 2

LiCPA genomic organisation and expression. A) Map of LiCPA locus with the different subclones analysed indicated. B) Southern blot of L. infantum JPCM5 gDNA hybridized with the LiCPA PCR product. The gDNA was digested using Sph I (lane 1), Pst I (lane 2), Xho I (lane 3), Sma I (lane 4), Sac I (lane 5) and Apa I (lane 6). C) Northern blot of total RNAs from L.infantum JPCM5 log phase promastigotes (P), stationary phase promastigotes (S) and axenic amastigotes (A) hybridized with the LiCPA ORF PCR product. After washing, the membrane was exposed on a phosphor-activated screen. An ethidium bromide-stained gel of RNA samples for each life cycle stage is shown in the lower panel.

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