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Figure 8 | BMC Molecular Biology

Figure 8

From: Characterizing exons 11 and 1 promoters of the mu opioid receptor (Oprm) gene in transgenic mice

Figure 8

PCR saturation studies. Saturation curve for each set of primers with different PCR cycles was established as described in the Methods section. In brief, PCRs with the indicated primer sets and cycle numbers were performed by using templates from a series of dilution of the known concentration of plasmids containing the target sequences. The plasmid concentration was ranged from 0.001, 0.01, 0.1, 1, 10, 100, 1000 and 10,000 amole. Relative band intensities from the ethidium bromide-stained gel were quantified with AlphaEase FC software. The linear phase of amplification for Exon 11 and Exon 11/tau targets (0.01 – 10 amole), Exon 1 and Exon 1/tau targets (0.1 – 100 amole), and G3PDH target (1~10,000 amole) was used to establish the linear regression equation using Prism 4.0. The sample concentration was calculated from the linear regression equation. The curves represented from three to six independent experiments. Each individual spot represents a single sample from the indicated primer set. Only part of the samples is displayed.

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