Figure 2From: Characterizing exons 11 and 1 promoters of the mu opioid receptor (Oprm) gene in transgenic miceSouthern blot analysis. Southern blot analysis was performed as described in the Methods section. Briefly, 5 μg genomic DNA from two transgenic lines, D13 and D10, was digested with BamHI, separated on 0.8% agarose gel and transferred onto a GenePlus membrane. The membrane hybridized with the GFP probe was exposed to the Kodak BioMax MS film.Back to article page