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Table 2 Esc4 binds to Sir3 in the two-hybrid system

From: Rtt107/Esc4 binds silent chromatin and DNA repair proteins using different BRCT motifs

   Interaction in two-hybrid strainsa
LexA hybrid GAD hybrid L40 L40 Δ sir3 L40 Δ sir4
LexA-Esc4 (818–1070) GAD-Sir3 (252–978) ++ ++ ++
LexA-Esc4 (818–1070) GAD-Sir4 (839–1358) + - +
LexA-Esc4 (818–1070) GAD vector - - -
LexA-Esc4 (1–480) GAD-Slx4 (383–748) +++ +++ +++
  1. a Three +++ signs means an easily visible blue color from the lacZ reporter gene was visible within 15 minutes, whereas two ++ signs means it was visible within 30 minutes and one + sign means it was visible between 40 minutes and two hours.