Rtt107/Esc4 binds silent chromatin and DNA repair proteins using different BRCT motifs

BMC Molecular Biology

Table 2 Esc4 binds to Sir3 in the two-hybrid system

		Interaction in two-hybrid strains^a
LexA hybrid	GAD hybrid	L40	L40 Δ sir3	L40 Δ sir4
LexA-Esc4 (818–1070)	GAD-Sir3 (252–978)	++	++	++
LexA-Esc4 (818–1070)	GAD-Sir4 (839–1358)	+	-	+
LexA-Esc4 (818–1070)	GAD vector	-	-	-
LexA-Esc4 (1–480)	GAD-Slx4 (383–748)	+++	+++	+++

^a Three +++ signs means an easily visible blue color from the lacZ reporter gene was visible within 15 minutes, whereas two ++ signs means it was visible within 30 minutes and one + sign means it was visible between 40 minutes and two hours.

ISSN: 1471-2199