Table 1 Yeast strains used in this study
From: Rtt107/Esc4 binds silent chromatin and DNA repair proteins using different BRCT motifs
Strain | Genotype | Reference or Source |
|---|---|---|
W303-1a | MAT a leu2–3,112 ura3-1 his3–11,15 trp1–1 ade2-1 can1–100 | R. Rothstein |
W303-1b | MAT α version of W303-1a | R. Rothstein |
W303 | Diploid from W303-1a × W303-1b | This study |
YDZ5 | W303 MAT a /MAT α esc4Δ::his5+/ESC4 | This study |
YDZ174 | W303 MAT a /MAT α esc4Δ::his5+/esc4Δ::his5+ | This study |
YDZ3 | W303-1a esc4 Δ::his5+ | This study |
YDZ4 | W303-1b esc4 Δ::his5+ | This study |
YEA78 | W303-1b hmr::URA3 HMR-E (aeb::G) gal4::LEU2 | This study |
YEA76 | YEA78 but HMR-E (Aeb::G) | This study |
YEA77 | YEA78 but HMR-E (aeB::G) | Thi study |
YAM7 | YEA76 sir2 Δ::kanMX6 | This study |
YEA118 | YEA76 but GAL4 sir3::LEU2 | This study |
YKAB17 | YEA76 sir4 Δ::kanMX6 | This laboratory |
YDS631 | W303-1b adh4::URA3-(C1–3A) n | [13] |
YDZ22 | YDS631 esc4 Δ::his5+ | This study |
YAM1 | W303-1a slx4 Δ::his5+ | This study |
YAM2 | W303-1b slx4 Δ::his5+ | This study |
YAM3 | W303-1a slx4 Δ::kanMX6 | This study |
YAM4 | W303-1b slx4 Δ::kanMX6 | This study |
YAM13 | W303-1b sgs1 Δ::kanMX6 | This study |
YDZ243 | W303-1_ sgs1 Δ::kanMX6 esc4 Δ::his5+ | This study |
YAM6 | W303-1a esc4 Δ::his5+slx4 Δ::kanMX6 | This study |
YAS501 | W303-1a asf1 Δ::his5+ | This laboratory |
L40 | MAT a his3 Δ200 trp1–901 leu2–3,112 ade2 URA3::(lexAop)8-lacZ LYS2::(lexAop)4-HIS3 | [54] |
YRJ3 | L40 sir3 Δ::kanMX6 | This study |
YJL103 | L40 sir4 Δ::kanMX6 | This study |