Figure 4
From: Rtt107/Esc4 binds silent chromatin and DNA repair proteins using different BRCT motifs
Targeted silencing activity is achieved by the C-terminus of Esc4. Three different regions of Esc4, containing either the first four BRCT motifs (1–480), linker region (480–836), or last two BRCTs (818–1070) were tested for targeted silencing in a strain with an HMR-E Aeb::G silencer and URA3 reporter gene at HMR. GBD-Esc1 served as a potent positive control and GBD as a negative control. SIR2-dependence of targeted silencing was also determined.